Human tartrate-resistant acid phosphatase (TRACP) ELISA Kit

FOR RESEARCH USE ONLY. Not for clinical diagnosis use For the quantitative determination of Human TRACP concentrations. Reactivity: Human Methode Type: Sandwich ELISA Detection Quantity: 96 tests Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates Detection range : 10 pg/L-400pg/L Sensitivity: 0.1 pg/L Components: Assay plate (12 × 8 coated Microwells) 1 Standard:450 pg/L 1×0.5ml Standard Diluent 1×1.5ml HRP-Conjugate Reagent 1×6ml Sample Diluent 1×6ml Chromogen Solution A 1×6ml Chromogen Solution B 1×6ml Stop Solution 1×6ml Wash Solution 1×20ml×30 fold User manual 1 Adhesive Strip 2 Product Principle: The kit is for the quantitative level of TRACP in the sample, adopt purified Human TRACP antibody to coat microtiter plate, make solid-phase antibody, then add TRACP to wells, Combine TRACP antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of TRACP in the samples is then determined by comparing the O.D. of the samples to the standard curve.